Imagej Add Scale Bar To Stack. Text and lines issues with adding text. If your image is already scaled pixels are displayed in µm values then skip to part 2.
Imagej is a useful tool for adding scale bars to images without much effort but after extensive searching i was unable to find a script that would add scale bars to large groups of images. This plugin points to the native menu command analyze tools scale bar. Asked 5 years ago.
Check the label all slices box to add a scale bar to the whole stack.
Define the units of length in the unit of length field. 5 now open the image you want to add a scale bar to. If your image is already scaled pixels are displayed in µm values then skip to part 2. I have taken a z stack of a cell using a zeiss confocal microscope.