Image J Western Blot Quantification. I used imagej and quantity one. Densitometry gel gel quantification imagej western blot.
The same procedure can be used to quantify dna or rna bands on agaros. This video shows you how to quantify bands on an image of an sds page gel using imagej. If you re looking for a more comprehensive workflow option for your western blot analyses.
Using imagej to quantify gel images this is a quick tutorial abour using imagej to process gel images taken with the geldoc.
The results of western blots can be assessed visually by making comparisons between bands in different lanes. The results of western blots can be assessed visually by making comparisons between bands in different lanes. The quantification will reflect the relative amounts as a ratio of each protein band relative to the lane s loading control. I am currentlly trying to get quantitative results out of my western blot films.